乌骨鸡黑色素细胞体外原代培养及鉴定

doi:10.11843/j.issn.0366-6964.2015.06.010

Abstract

Abstract:

The purpose of this study was to establish a method for melanocytes culture and identification system in vitro，and to provide an ideal biological material for investigating the melanocytes functions.Peritoneum of Silky Fowl was collected and digested by dispaseⅡ and trypsin-EDTA mixed digestive solution under different digestive time.The pure melanocytes were obtained after continuous passages.Through the observation of cell morphology and cell growth curve showed that the primary melanocytes grew well under the specific medium.The cell specificities were further confirmed by the morphology，ultrastructure observation under electron transmission microscope，genes expression associated with melanin synthesis，and cell characteristic by DOPA stain and immunocytochemistry.The successfully cultured melanocytes sustained the normal biological characteristics on passage 10 demonstrating that the culture of primary melanocytes was accomplished under this in vitro system.All together，the in vitro culture system of melanocytes from Silky Fowl was successfully established in our study.

CLC Number:

S813.3

HAN De-ping，WANG Shu-xiang，ZHANG Yuan-yuan，YANG Zu，LI Jun-ying，DENG Xue-mei. Culture and Identification of Primary Melanocytes from Silky Fowl in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 949-956.

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Culture and Identification of Primary Melanocytes from Silky Fowl in vitro

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